Ana Krstajić was born in Belgrade. She graduated from the Faculty of Music Arts, Department of Composition and Orchestration (M.M. Serbia), and from Berklee College of Music, Department of Film, TV, and Video Game Scoring (M.M. Spain).

In 2014, she won 1st prize at the young composers' competition "Women's Works" (Ithaca, New York). In 2016, she won 2nd prize at the International Composer Competition "De Bach au Jazz" (Paris, FR) in the Contemporary Piano category, and that same year, she won 1st prize at the International Music Competition "The Best Musical Mind" (Belgrade, SR), which earned her a full scholarship for the Berklee Summer Performance Program (Valencia, ESP). She received an Honorable Mention at the Soundtrack Cologne Festival in Cologne in 2018, and in 2021, she won the Hollywood Music in Media Award (Los Angeles, California) for her composition "Naša zemlja" (Our Land). In 2022, she won the Score Live competition and was awarded a masterclass in film music in Madrid, where she recorded her work with the Spanish Radio and Television Orchestra. She was awarded the Muzika Klasika prize for the best applied music composer in 2023. Since 2019, she has been a cultural ambassador for Serbia Creates. She was selected for a mentoring session by the Alliance for Women Film Composers and the Society of Composers and Lyricists in America. She is currently working on several film, TV, and theater productions in Serbia and composing music for a collection of short animated films for Apple TV. She is also the resident artist at Kolarac Endowment in Belgrade.